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Bioengineering tutor required who can answer practice questions and explain the answers -- 2

I am looking for a Bioengineering tutor who can teach me for my exam. You will need to answer many practice questions and explain me the answers accordingly. The sample questions are given below. If you are expert tutor in this field, you may apply-

Condition: Prakhar is a M.Sc. student who is working on the human gene, insulin, and wish to produce the protein for therapeutic purposes. Help him purify the protein where he has used Nterminal His-tagged-gene sequence to facilitate the protein purification process.

Guide Prakhar to choose the correct expression host. Which of the following features should be considered to choose the correct host?

Length of gene

Presence of introns

Post translational modifications

Toxicity to the host cells

Prakhar selected E. coli system for protein expression but when he grew the culture with pET28a expression vector, he could see low growth of the bacteria. He transformed the recombinant vector in the expression host with pLysS plasmid. The transformed plates are shown in the image below. Which of the following is FALSE for the observation shown below?

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A is E. coli strain with GOI and pLysS plasmid

B is E. coli strain with GOI and pLysS plasmid

pLysS helps in debilitating the leaky expression of GOI

Protein product of GOI is toxic to host cells

The opted chromatography was conducted and elute was collected. Refer to the image help Prakhar to understand the possible reasons why the eluent’s profile looks like this?

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Where, Lane 1 – Ladder, Lane 2 – Lysate, Lane 3 – FT, Lane 4 – Wash I_1, Lane 5 - Wash I_2, Lane 6 – Wash II_1, Lane 7 – Wash II_2, Lane 8 - Wash II_3 and Lane 9 – Elution1

N-terminal His-tag might lead to production of truncated protein

The elute can be a multimeric protein

Bacterial protein with high histidine content

a and c

Prakhar wish to confirm each band if it contains the desired protein of interest. Help him to select correct technique(s) to confirm the correct band.

Mass spectrometry

Western blotting

Microarray

ELISA

Post conducting the 1st chromatography Prakhar observed that the purified elution contains four prominent bands at size difference of at least ~10 kDa. Which of the chromatography methods can he use to obtain pure protein?

Reverse phase chromatography

Ion exchange chromatography

Affinity chromatography

Size exclusion chromatography

Prakhar needs to confirm the sequence of the recombinant plasmid he prepared. He used Sanger’s Sequencing of DNA molecules. Which of the following is correct for the sequencing method?

4’OH present, extends an already present DNA fragment, give radioactive signal

4’OH present, terminates DNA strand from extension, gives fluorescence

3’OH absent, terminates DNA strand from extension, gives fluorescence

3’OH absent, extends a new DNA fragment, gives chemical signaling

Prakhar isolated the recombinant plasmid for sequencing and diluted the sample with a dilution factor of 1:40. The UV-Vis Spectrophotometer Absorbance reading at 260 and 280 nm obtaining 0.42 and 0.21 readings respectively. So, calculate the concentration of plasmid in the dilute and concentrated plasmid samples.

DNAconc. in plasmid = 21 ng/ml; DNAconc. in plasmid diluent = 67.2 ng/ml

DNAconc. in plasmid = 21 μg/ml; DNAconc. in plasmid diluent = 67.2 μg/ml

DNAconc. in plasmid = 21 ng/ml; DNAconc. in plasmid diluent = 0.52 ng/ml

DNAconc. in plasmid = 21 μg/ml; DNAconc. in plasmid diluent = 0.52 ug/ml

Fähigkeiten: Biotechnologie, Biomedical Engineering, Mikrobiologie, Biologie, Ausbildung u. Privatunterricht

Über den Kunden:
( 0 Bewertungen ) Berlin, Germany

Projekt-ID: #32949232

1 Freelancer bietet im Schnitt €15 für diesen Job

huzaifarizwan995

I will provide solutions for both in-plant and domestic problems faced in chemical and process engineering industries. My subject expertise is in heat and mass transfer, fluid dynamics, unit operations, reactor enginee Mehr

€15 EUR / Stunde
(2 Bewertungen)
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